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Technology of wearable sensors
Javor, Adrián ; Majzlíková, Petra (referee) ; Hubálek, Jaromír (advisor)
This bachelor thesis deals with thick-film and thin-film technology of creating wearable sensors on human body, that sense substances in perspiration. Part is devoted to their classification and making subsequent proposal of flexible three-electrode biosensors using 4 different electrochemical transducers. All of these biosensors sense concentration of lactate in human sweat, and are based on immobilization lactate oxidase enzyme on the working electrode. Technological process of production is based on combination of thick-film and thin-film technology. Conclusion of this bachelor thesis contains amperometric evaluations of the lactate concentration, from which calibration curves were created.
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Utilization of lactate during exercise in the context of genetic dispositions
Vostatková, Pavlína ; Petr, Miroslav (advisor) ; Heller, Jan (referee) ; Balkó, Štefan (referee)
Title: Utilization of lactate during exercise in the context of genetic dispositions Objectives: The aim of the dissertation is to investigate, within a group of athletes from speed- endurance disciplines, the influence of selected polymorphisms in genes encoding lactate transporters MCT1-4 on lactate levels before, during, and after Intermittent 30s Wingate Test. A sub-objective of the study is to identify differences in allele and genotype frequencies in the respective polymorphisms between the group of athletes and controls. Methods: The research group consisted of 91 Eastern European Caucasian men, members of the Czech Athletic Association with elite performance in the 400 m run (age 20.4 ± 3.2 years; body weight 74.07 ± 7.5 kg; height 182.7 ± 6.14 cm). A control group (n=100; age 20.5 ± 1.2; body mass 77.5 ± 9.47 kg; height 181.5 ± 6.35 cm) of physically active individuals who did not perform speed and strength sports was used for allele and genotype comparisons. For genotypic analysis of selected polymorphisms, buccal mucosa swabs were performed in the probands. DNA isolation and subsequent genetic analyses were performed in collaboration with the Józef Piłsudski University of Physical Education in Warsaw. The frequencies of individual alleles and genotypes were tested for compliance with...
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Comparison of body composition and physical level of students of the Military Branch at FTVS UK at the beginning and at the end of their studies.
Gacek, Tomáš ; Sovák, Libor (advisor) ; Oláh, Vladan (referee)
Title: Comparison of body composition and physical level of students of the Military Branch at FTVS UK at the beginning and at the end of their studies. Objective: To evaluate the results from the measurements of the members of the VO FTVS UK at CASRI, what changes occurred in terms of their physical performance and body composition in the beginning and in the end of their studies. Method: Paired t-test was used to analyze the data of members of the VO FTVS UK who successfully completed full-time undergraduate and graduate studies. These were data from 8 males (age 24,3±1,5; weight in kg 79,3±6,1; height in cm 179,5±5,8) and 1 female (age 24; weight in kg 66,9; height in cm 171) from measurements at CASRI, which tested physical level on a stepwise test (on a treadmill) and body composition on the TANITA MC-780. Results: Paired t-test analysis of the data confirmed a statistically significant difference in muscle mass gain (mean difference=3,14 kg; p=0,0007) at the end of the study. On the other hand, during the stepwise test, there was no statistically significant difference in final heart rate (mean difference=0,58; p=0,9394) or lactate levels (mean difference=0,66; p=0,1639). Based on this measurement, it can be concluded that there was no statistically significant deterioration in physical level...
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Bakteriální kontaminace rybích produktů - mikrobiologický a MALDI TOF přístup
GÁPOVÁ, Pavlína
This diploma thesis was focused on microbial contamination of fish meat. Common carp (Cyprinus carpio) was chosen as a model fish. The work aimed to optimize the conditions for bacterial growth using four different growth media and three different temperatures. Incubation took place at 0, 72 and 120 hours after killing the fish and the samples were incubated at 0, 4 and 8 ° C. Agars selected from a broth of common carp (Cyprinus carpio), grass carp (Ctenopharyngodon idella), rainbow trout (Oncorhynchus mykiss) were used as growth media and a commonly used bovine-based agar was added as a control. After incubation, the obtained bacterial colonies were analyzed by MALDI-TOF MS. At the same time, two chemical analyzes were performed on other meat samples stored under the same conditions. The first was to determine how the content of ATP and its metabolites (Hx + R and Hx) will change over time. The second analysis focused on the change in lactate content over time. The work was divided into 4 phases. The most demanding phase of the whole project, both time and manual, was the preparation of samples for testing and MALDI-TOF MS. The content of ATP and lactate in the meat was detected by high-performance liquid chromatography-ultraviolet (HPLC-UV). The results of the work did not show the influence of the composition of the growth medium on the final microbial community. Temperature proved to be the most significant factor influencing the growth of microorganisms. Bacteria demonstrably grew more and faster at higher temperatures. The lowest and slowest growth was recorded for samples growing at 0 ° C. The second important factor, mainly influencing the species composition of the bacterial community, was the time from the killing of the fish to the time of testing. While the community was grown on the media vaccinated at 0 and 72 hours after slaughter was relatively diverse, the media vaccinated at 120 hours after slaughter have already succeeded in isolating almost exclusively Pseudomonas spp. Analyzes of the content of ATP and lactate have shown that at lower temperatures - respectively the temperature of 0 ° C there is not only a significant slowing down of bacterial growth but also a slowing down of the autolysis process. Differences in storage at 4 and 8 ° C were no longer significantly different.
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Influence of cold water immersion on repeated strength endurance performance in football players
Šíma, Marek ; Jebavý, Radim (advisor) ; Blažek, Dušan (referee)
Title: Influence of immersion in cold water on repeated strength endurance performance in football players Objectives: The aim of this study was to assess the acute effects of cold water immersion on repeated strength endurance performance in the form of 30 maximum vertical jumps in football players. Methods: The effect of immersing the lower limbs to the level of the navel in cold water on repeated strength endurance performance in the form of CMJ (countramovement jump) in football players. Twenty-two football players (men) performed an entry and exit strength endurance test in the form of vertical jumps on the Kistler board. Between the input and output performance tests, two cold water immersion regeneration procedures (CWI) and passive rest (PAS) were applied. For the evaluation of force endurance performance, data on jump height (cm), force impulse (Ns), flight time (ms), speed (m / s), force (N), force / weight (N / kg), power ( W), power / weight (W / Kg). As a marker of fatigue, blood lactate levels (mmol / l) were monitored, we also monitored skin temperature (ř C) during CWI and subjective feeling of fatigue (1-10). The influence of two types of regeneration procedure and two strength endurance performances were evaluated using ANOVA. Results: No statistically or materially significant...
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Metabolic Effects Of Hyperthermic Isolated Limb Perfusion (HILP) in Malignant Melanoma Patients
Hodková, Gabriela ; Semrád, Michal (advisor) ; Štádler, Petr (referee)
Hodková, Gabriela - Metabolic Effects Of Hyperthermic Isolated Limb Perfusion (HILP) in Malignant Melanoma Patients First Faculty of Medicine, Charles University in Prague, Praha 2, Kateřinská 32 Head of the work: Doc. MUDr. Michal Semrád, CSc. Supervisor - consultant: MUDr. Miroslav Špaček, Ph. D. The aim of the study is to assess the metabolic consequences of mechanical isolation and hyperthermic cytostatic perfusion in a limb affected by malignant process. The theoretical part refers to a topic of malignant melanoma, its clinical evaluation and treatment. Methods based on conservative and surgical treatment are described. The isolated hyperthermic cytostatic limb perfusion is a consecutive local treatment indicated in cases of recurrent malignant lesions following surgical resection, when next surgery is impossible. In the practical part, the laboratory samples and clinical data were recorded in patients who had undergone hyperthermic cytostatic limb perfusion in the 2nd Surgical Department of The General Teaching Hospital and First Faculty of Medicine, Charles University Prague. The affected limb was flushed with a warm oxygenated blood containing cytostatic drugs using an extracorporeal circuit apparatus. Selected arterial blood gas, metabolic and hematologic parameters were studied intra and...
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The aplication of CSS in swimming flume
Cagaň, Štěpán ; Jurák, Daniel (advisor) ; Chrzanowská, Babeta (referee)
Title: Application of CSS (Critical Swimming Speed) in the swimming flume. Objectives: Purpose of our pilot study was to find out, if load during swimming on speed of anaerobic threshold in the flume on level validated by accredited workplace corresponds with load during swimming in the swimming pool. Methods: For verification of our purpose, for each proband we first determined speed of anaerobic threshold, using Critical Swimming Speed protocol. Subsequently we monitored values of blood lactate and heart rate during swimming on anaerobic threshold speed level in the pool and thereafter in the flume. We have compared measured values. Results: We found out, that load in the flume is much lower, than load in the pool. We also found out, that stream of water in flume is not constant. Keywords: flume, swimming, swimming simulator, lactate, front crawl stroke, critical swimming speed
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Testing of maximum SF in a swimming flum, and swimming pool - a comparison of methods
Hubička, Tomáš ; Jurák, Daniel (advisor) ; Čechovská, Irena (referee)
Title: Testing of Maximum HR in the Swimming Flum and Swimming Pool Objectives: The aim is to compare the detection method of max HR in a swimming Flum with the method of detection max HR in the classic swimming pool. By meeting other goals we want to determine which method is more precise, what the positives and negatives of individual measurements show and what the difference are practically max values observed in HR pool and the Flume compared with the results of theoretical calculation max HR. Methods: According to a pilot study with methodology of the thesis, HRmax was tested in a swimming Flum and the methodology according to Formanek and Horčic HRmax testing was carried out in the swimming pool. A sample of probands were selected from students UK FTVS. For the recording was used sporttester and data evaluation program execution Polar Precision Performance. To determine the submaximal swimming speed we used CSS test. This rate was also used to warm up before swimming test to the individual HRmax of probands in a swimming Flum. The measured data of both measurements were compared with each other and the results described and evaluated. Results: CSS gained speed in m/s, was used in the Flume in escalations test to HRmax. We measured values HRmax tests in a Flume and in a swimming pool. The Test...
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Effects of single beta-alanine dose on performance at submaximal load
Šimsa, Jáchym ; Sýkora, Karel (advisor) ; Šteffl, Michal (referee)
Title: Effects of single beta-alanine dose on performance at submaximal load Objectives: The aim of this study was to investigate the effects of single beta-alanine dose on blood-lactate level in capilary blood and whether it will affect the speed of passing the test. Methods: A double-blinded cross-over study conducted on 16 male students of Military Department at the Faculty of Physical Education and Sports of Charles University (mean ± SD: weight 79,31 ± 6,97 kg; height 181,56 ± 6,36 cm; age 21,63 ± 1,45 yrs). The study was performed in Military Sport Centre Pilotů 217/12, 161 00, Prague. Each volunteer underwent two performance tests after consuming either placebo (maltodextrine) or beta-alanine (50 mg/kg). Time needed to complete the test and blood-lactate concentration before and during 5th and 10th minute after completion was measured. Volunteers also filled a short questionnarie regarding subjectively percieved load and side effects, and whether they were administered beta-alanine (BA) or placebo (PLA). For verification of normal distribution were used Q-Q Plots and statistical significance of BA use was determined using student's t-test. Results: Measured lactate concentrations were (mean ± SD; mmol/l) for BA in min 0 = 1,29 ± 0,37; min 5 = 11,48 ± 1,45; min 10 = 10,64 ± 1,76; PLA in min 0...
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Comparsion of the blood lactate level of an elite judokas during training and competition loads
Ječmínek, Jan ; Coufalová, Klára (advisor) ; Pavelka, Radim (referee)
Title:
Comparsion of the blood lactate level of an elite judokas during training and competition loads Objectives: The aim of this study was to compare the lactate level of an elite judokas during training and competition loads and to identify potential relationships with other entering variabilities. Methods: The chosen method was empirical, ie collecting data from its own research and statistical representation of the data and linked to the context with other studies. Results: It was found that lactate levels during competition load increases dramatically over training load and pulse rate has no statistical significance as an indicator of the accumulation of lactate. Key words:
Judo, lactate, training, competition.
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